ASGSB 2003 Annual Meeting Abstracts
THE TRANSCRIPTIONAL NETWORK OF GRAVITROPISM IN ARABIDOPSIS ROOT TIPS.
H. Winter Sederoff,
J. M. Kimbrough, R. Salinas-Mondragon, and C.S. Brown.
NSCORT, Dept. Botany, NC
response to environmental changes, cells in tissues of an organism go through
a specific program of temporally and spatially coordinated gene expression to
adapt to changing conditions. In Arabidopsis root tips, rapid, temporal
changes in the abundance of specific transcripts occur in response to
gravity-stimulation and these transcript level changes reveal clusters of
coordinating signaling events that are required for the tissue to make a
commitment to differential growth.
have carried out microarray analysis of Arabidopsis root tips after
gravistimulation (reorientation) and mechanical stress and monitored
transcript levels of ca. 24,000 genes in a time-course during the first hour
after either stimuli. We identified genes responding to either stimulus
specifically as well as transcripts regulated in both signaling pathways. The
temporal patterns of transcription and transcript stability of those induced
genes are very distinct. We also identified several previously hypothetical
genes that were induced during gravitropic stimulation (now named GIGs –
Gravity Induced Genes) and analyzed the network of transcriptional regulators
during the early stages of gravitropism.
(Supported by NASA: NAG2-1566)
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