ASGSB 2003 Annual Meeting Abstracts
REGULATION OF RAPID, TRANSIENT GENE EXPRESSION DURING GRAVITROPIC STIMULATION
J. M. Kimbrough, R.
Salinas-Mondragon, C.S. Brown, and H. Winter Sederoff NSCORT, Dept. Botany, NC
State University, Raleigh.
Gravity-specific transcriptional regulation occurs within less than 2 min in
an Arabidopsis root tip.
analysis revealed several “very early transcripts” that comprise a group of
genes coding for proteins functioning in the transcriptional and translational
machinery (transcription factors, ribosomal RNA, RNA binding proteins, DNA
binding proteins), some predicted (hypothetical) proteins and proteins with
unkown function, as well as some proteins predicted to be involved in signal
transduction and transcription.
Steady-state mRNA levels
reflect the relative rates of synthesis versus degradation of these specific
transcripts. Several of these very early transcripts were “up-regulated” and
several were “down-regulated”. Increased rates of transcription are usually
brought about by specific promoter elements, while 3’ and 5’ untranslated
regions (UTRs) are known to be involved in transcript stability. We have
analyzed the promoter sequences and UTRs of those transiently regulated
gravitropically regulated genes for known sequence elements and generated new
motifs possibly involved in the transient nature of the early gravitropic
(Supported by NASA: NAG2-1566)
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